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pcat enhancer vector  (Promega)

 
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    Promega pcat enhancer vector
    Pcat Enhancer Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcat enhancer vector/product/Promega
    Average 90 stars, based on 1 article reviews
    pcat enhancer vector - by Bioz Stars, 2026-06
    90/100 stars

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    Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, pCAT®3-Enhancer vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.

    Journal: Immune Network

    Article Title: A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity

    doi: 10.4110/in.2009.9.6.243

    Figure Lengend Snippet: Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, pCAT®3-Enhancer vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.

    Article Snippet: Several promoter regions used in the dual reporter vector were re-cloned into the CAT expression vector system pCAT®3-Enhancer vector (Promega, Madison, WI), in order to ascertain whether these regions evidenced similar transcription activity in the CAT assay system ( ).

    Techniques: Plasmid Preparation, Clone Assay, Chloramphenicol Acetyltransferase Assay, Activity Assay, Sequencing